Cooking in 20 ml vial

[smithmitchelle]

Is it safe to use a 20 ml sterile vial to boil the oil before adding powder in.

[waderow]

you need to filter it after its mixed anyways, so doesnt matter

[natenator]

BOIL oil? Those words should never go together!

[smithmitchelle]

haha well the cotton seed oil is sterile and and the vials are also sterile from board source, my powder is lab quality and sealed. Hypothetically everything is sterile and would not require filtering/cooking.

I know some people would be against not filtering it but Ive heard also if your gear is clean filtering is not required. (trusted and not a ugl)

or should I go order a whatman just to be safe?

[waderow]

filter and re-sterilize

[natenator]

in a oven proof glass container:

put in oil
put in hormone
put in BA
Put in BB

baked in over at 250 for 25 mins or until clear and no swirls.

Place 22G pin in vial (to relieve pressure)
Attach filter to a pin and stick in vile
draw oil into a syringe, attach to filter and push (repeat to all oil is filter and in sterile vial)


that's it.

MODS: If this post is wrong please delete it.

[Beachmuscle]

I think your next post is going to be about an abcess. You need to do a lot more RESEARCH.

[smithmitchelle]

Well its not like Im trying to shoot the shit here... is this not considered research?
asking my fellow cbb's for advice?

no offense but I prefer constructive criticism and helpful advice. Thanks to natenator and waderow for advice.

now I need to get a whatman filter somewhere and I dont want make a whole order to CMS just for a filter. Any ideas around the Toronto area?

[monkey]

:laugh
I think your next post is going to be about an abcess. You need to do a lot more RESEARCH.

[Blitz-Test]

So you think powder from a third world country is sterile

[smithmitchelle]

reliable source, non-ugl. Either way I am taking caution and using a filter.

[Blitz-Test]

good choice man, you gotta think most of these countries where powders are made havent even figured out that you shouldnt have a waste management disposal unstream of your city drinking water...

[CBB loves Japan]

if you're not going to filter at least add a bit extra ba, say 5% and be sure to let i sit sealed 2 weeks to kill anything.

You still run the risk of injecting cell wall material of lysed bacteria that may have gotten in.

Though so few labs have microbiological experience, the correct way to maintain sterility is dissolve powders in bb, WITHOUT ba yet, filter that to remove all whole live cells, then add ba to act as a preservative, not a sterilizing agent. Too many labs run by non-scientists believe filtering a finsihed product is good practice, when in fact the most toxic cell fragments including LPS can slip through .22 quite easily. Adding ba initially is a huge mistake no educated lab owner would make. Though unfortunately the truth is most are not. I kew the owner of one lab that was very popular on nexus and he cooked oils ina frying pan.

Business major 20 year old kid. MAjor lab.

[Merc63]

if you're not going to filter at least add a bit extra ba, say 5% and be sure to let i sit sealed 2 weeks to kill anything.

You still run the risk of injecting cell wall material of lysed bacteria that may have gotten in.

Though so few labs have microbiological experience, the correct way to maintain sterility is dissolve powders in bb, WITHOUT ba yet, filter that to remove all whole live cells, then add ba to act as a preservative, not a sterilizing agent. Too many labs run by non-scientists believe filtering a finsihed product is good practice, when in fact the most toxic cell fragments including LPS can slip through .22 quite easily. Adding ba initially is a huge mistake no educated lab owner would make. Though unfortunately the truth is most are not. I kew the owner of one lab that was very popular on nexus and he cooked oils ina frying pan.

Business major 20 year old kid. MAjor lab.

I'd love to know why filtering powder with just BB is going to make a difference? Also, why adding BA after is going to make a difference at all? If this bacteria is going to pass a .22 then what difference would it make if you dissolved the powder in just BB first?? That makes zero sense.

Are you implying to just filter the BB/powder and not the oil/BA? That's the most ridiculous thing I have heard.

[tiramisu]

Powders are not sterile for injection, they are for the most part simply chemicals from a warehouse and must be filtered. If you are lucky you are getting a pharmaceutical grade of chemical that has adequate purity for injection but there is no way that you can prove that your vendor has not saved himself the money and shipped you a lower grade of chemical (this is likely fairly common). The difference between grades in chemical pricing is generally fairly significant.

I use lab sterilized sealed vials, .2 micron whatmans, and adequate BA to keep the oil from being a breeding ground. So I don't generally worry about dry heat sterilization. I also don't feel comfortable that you can fill and crimp your own vials without contaminating the vials, little stoppers, crimping material or oil unless you have a sterile environment to work in. While I can certainly ensure a clean environment there is no way that it I can create a sterile one. I also don't see a need to engage in a really complicated process when I can use a simple one that is safer and more controllable.

... But that's just me.