IGF reconstitution in NaCl

[waderow]

There was some people who were under the impression that recon of IGF in NaCl or BW was acceptable, and that the peptide would not degrade.

I have recently tried IGF in NaCl and experienced degradation after 1 1/2 weeks.

Just thought I would share.

Recon in AA is necessary in my opinion, with a pre-jab dilution in NaCl.

[AlbertaBeef]

How could you tell it degraded after 1.5 wks? Just curious
I agree though, unless you have 100mcg amps AA is the way. I've heard guys use BA as well but I'm skeptical about it.
I've been mixing and then add NaCl for shots because I hear it impedes necrosis where BW doesn't.

[waderow]

How could you tell it degraded after 1.5 wks? Just curious
I agree though, unless you have 100mcg amps AA is the way. I've heard guys use BA as well but I'm skeptical about it.
I've been mixing and then add NaCl for shots because I hear it impedes necrosis where BW doesn't.

lost the feel mid vial. lost the glorious pumps. you can just tell.... i have used with aa and never had this problem. the igf is fresh, and good without a doubt.

[gustavo77]

The alcohol in NaCl or Bateriostatic water will definitely destroy the very fragile IGf-1 peptide...always dilute the AA/IGf-1 immediately prior to injection and also make sure to always draw the AA/IGf-1 first. Never let any NaCl or B water come into contact with the IGf-1 vial.

[waderow]

the IGF will survive in the syringe in a diluted state for at least 2 hours. i have preloaded slin pins, worked out, and then PWO jabbed with great results.

But yes, draw your IGF, and then your dilutant as not to contaminate vial

[gustavo77]

the IGF will survive in the syringe in a diluted state for at least 2 hours.

Good to know bro, thanks!

[MassNutrition]

Quick question... I have reconstituted 1mg of IGF with 2ml of AA, and I of course, dilute it with NaCl before sticking myself. My question is, how long will my IGF be good for in its reconstituted state with the AA?

[waderow]

Quick question... I have reconstituted 1mg of IGF with 2ml of AA, and I of course, dilute it with NaCl before sticking myself. My question is, how long will my IGF be good for in its reconstituted state with the AA?

my last vial was 5 weeks with punch right until the end in AA.
I have been told a year refridgerated.

The biggest thing is make sure yo ukeep it in the fridge.

Also, why so much AA?

[MassNutrition]

Also, why so much AA?

I figured it was a good number to do the math with... If I wanted 50mcg per shot, all I did was draw 10IU in an insulin syringe (gave me 20 shots), then dilute it with another 10IU of NaCl directly in the syringe...

...any suggestions on how I could do it differently in the future?

[waderow]

if youre doing 50's i suppose it is more accurate to pull to 10 instead of 5. I am doing 100mcg so 1cc of AA is lots. youre fine as is.

[boboy]

I will use for the first time of the igf, and I would like to know which are best proportioning for a beginner. Thank you

[Rrrrolla]

Where do you guys get your acetic acid? What is a good ratio of acetic acid to BW to shoot without hurting my delicate muscles? I have always has 100mcg vials, but now my guy ships 1mg vials.

Thanks!
R

[gustavo77]

Where do you guys get your acetic acid? What is a good ratio of acetic acid to BW to shoot without hurting my delicate muscles? I have always has 100mcg vials, but now my guy ships 1mg vials.

Thanks!
R

Bro if you have 100mcg vials there is no real need to use AA unless you are not going to use a 100mcg within a couple of days....You can simply use B water or Sodium Chloride to mix the IGF-1.

[Rrrrolla]

Ya, that's what I have been doing, but now I need to reconstitute 1mg vials. Changed suppliers.

[MassNutrition]

I personally dump 2cc of AA into my 1mg vials, which yields me 200IU of course. So all I do is draw 10IU at a time, and that gives me 50mcg per shot (20 shots total). I then stick my NaCl vial and draw out 10IU of that to dilute it, then jab 2 bodyparts...

I have been seriously considering trying 100mcg shots with this upcoming cycle. So what I might do is drop the AA to 1cc, which will yield 100mcg for every 10IU I draw out... dilute, jab, etc...

[gustavo77]

I personally dump 2cc of AA into my 1mg vials, which yields me 200IU of course. So all I do is draw 10IU at a time, and that gives me 50mcg per shot (20 shots total). I then stick my NaCl vial and draw out 10IU of that to dilute it, then jab 2 bodyparts...


This is similar to what i do only i use 4ml of AA giving me 25mcg/10iu on a 1cc slin pin.

[Rrrrolla]

So where do we get AA and NACL?

[bigdaddydrew123]

i know a guy who used vinegar to recon igf . its about 5 % aa.dont think its a good idea . the look of pain on his face was priceless tho

[gustavo77]

So where do we get AA and NACL?


K, the AA needed for IGf-1R3 recon is 100mM. White vinegar is 5% AA or 850mM...you need to get it to 100mM, so you need to mix it with sterile water.

Buy a new bottle of white vinegar and get some sterile water (no preservatives). NOT bacteriostatic water or sodium chloride!. You can get the sterile water from the pharm, it is for one single use, so toss it after you do this...once mixed with the vinegar the vinegar will serve as a preservative. To mix up 10ml of AA suitable for IGF-1 recon, simply, in a sterile vial mix 1.2cc of vinegar and 8.8cc of sterile water, which will bring it to approx 100mM...now you have 10ml of AA, enough for 5-10mg depending on how much you use for recon..i use 4ml, that gives 25mcg/10units on a 1cc slin pin. Many people say you should use a whatman syringe filter (0.20u) to filter your vinegar and water while mixing...definitely a good idea.

[MassNutrition]

for recon..i use 2ml, that gives 25mcg/10units on a 1cc slin pin.



Bro, I think that calculation may be wrong. I recon my stuff the same way... 10 units on a slin pin should yield you 50mcg, not 25mcg. Here's my math:

1000mcg/200iu (2ml) = 5mcg/iu

Therefore, drawing out 10iu will give you 50mcg.

You just said 4 posts up that this was exactly what you did LOL...

[Mr.Freeze]

the sponsors as it i think:confused: Canadian medsupplies

[gustavo77]

Bro, I think that calculation may be wrong. I recon my stuff the same way... 10 units on a slin pin should yield you 50mcg, not 25mcg. Here's my math:

1000mcg/200iu (2ml) = 5mcg/iu

Therefore, drawing out 10iu will give you 50mcg.

You just said 4 posts up that this was exactly what you did LOL...

You are correct bro, i actually put 4ml of AA into my 1mg vial...giving me 25mcg/10iu...my mistake..

[MassNutrition]

K, the AA needed for IGf-1R3 recon is 100mM. White vinegar is 5% AA or 850mM...you need to get it to 100mM, so you need to mix it with sterile water.

Buy a new bottle of white vinegar and get some sterile water (no preservatives). NOT bacteriostatic water or sodium chloride!. You can get the sterile water from the pharm, it is for one single use, so toss it after you do this...once mixed with the vinegar the vinegar will serve as a preservative. To mix up 10ml of AA suitable for IGF-1 recon, simply, in a sterile vial mix 1.2cc of vinegar and 8.8cc of sterile water, which will bring it to approx 100mM...now you have 10ml of AA, enough for 5-10mg depending on how much you use for recon..i use 4ml, that gives 25mcg/10units on a 1cc slin pin. Many people say you should use a whatman syringe filter (0.20u) to filter your vinegar and water while mixing...definitely a good idea.

I went to 3 different Shopper's DMs and asked for the sterile water... I got funny looks by everyone. No one carries sterile water apparently...

...is this the same as distilled water? Does it have to be for "injection"? I tried to explain that it had to have NO preservatives in it. They asked what it was for and I didn't know how to explain myself. So one guy hands me a 10ml SINGLE DOSE vial of 0.9% Sodium Chloride. It says "diluent - no preservative" on it. Is this shit ok to use? If not, what EXACTLY am I looking for and how do I ask for it? Thanks Bros...

[waderow]

I went to 3 different Shopper's DMs and asked for the sterile water... I got funny looks by everyone. No one carries sterile water apparently...

...is this the same as distilled water? Does it have to be for "injection"? I tried to explain that it had to have NO preservatives in it. They asked what it was for and I didn't know how to explain myself. So one guy hands me a 10ml SINGLE DOSE vial of 0.9% Sodium Chloride. It says "diluent - no preservative" on it. Is this shit ok to use? If not, what EXACTLY am I looking for and how do I ask for it? Thanks Bros...

NO NaCl will degrade IGF.

Use distilled water from supermarket and run through sterile syringe filter

[Grunt76]

Benzyl alcohol is no better than BW or NaCl for reconstitution. Its only advantage is that it allows the IGF-1 to be kept in the freezer without the alcohol turning solid and possibly hurting the peptide. The lower temperature makes for a slower degradation rate.

But compare that with 100mM AA, where even at room temperature, laboratory measurements of degradation are nil even after 2 years... Yeah. AA it must be.