Mastagon
15-09-2009, 01:19 AM
When I first started researching how to reconstitute IGF, I never thought it would take 10 hours or so of looking over 4 different boards to come down to something definitive. There are so many compounds, combinations, % strength, acidity, etc that people use for this peptide it was ridiculous. Maybe all this is easy if you are a chemist or have "been in the game" a while, but I found it to be a bitch. ANYWAY. Turns out the best recon mix is acetic acid .6% strength. This mixture will ensure the IGF (IGF-1, IGF-1-LR3, IGF-II or otherwise) is stable at room temperature for a year or more, and is exactly the kind of thing for people who don't plow through 1mg bottles in a week's time.
And the best thing about .6% acetic acid? You can make it yourself:
Things you will need
distilled 5% white vinegar (grocery store, yes, the stuff you put on fish and chips)
distilled water (grocery store, pharmacy)
Alcohol prep pads – sterile kind (70% isopropyl alcohol, pharmacy)
Equipment:
two .2-.22 sterile syringe filters (whatman and millipore are common types)
10mL syringe with a luer lock tip (smaller syringes can obviously work, though you'll be drawing more frequently)
1 standard sterile new syringe
2 new and sterile 20-22 gauge needles
1 empty Sterile glass vial (10-20mL)
Procedure: Alot of this is common knowledge, but here it is anyway
1. swab the top of the empty sterile vial with the alcohol pre pad
2) put the syringe filter on, then attach the needle to this filter. draw up 7.5ml distilled water, then 1ml of vinegar
3) dispose of this needle and filter, attach a new syringe filter, attach the needle onto this filter (making sure not to touch the end of the filter or the exposed needle with your fingers)
4) Inject the 8.5mL of solution into the sterile vial
5) the syringe filter ensures that the mixture you've put in that vial, and
eventually into your body, will be sterile. Using a filter for drawing AND for injecting might be overkill, but it never hurts to be cautious.
5) you now have 8.5ml of of .6 acetic acid
The Reconstitution itself
1) get the regular new sterile syringe
2) draw up the desired amount of .6% aa, which would depend on how strong you want your IGF (read: mcg/ml or mcg/iu)
3) swab the top of the vial containing the IGF
4) inject the mixture SLOWLY down the side of the vial (never directly onto the powder itself!)
5) gently swirl the mixture around until uniform
6) There, that's it.
Notes:
You might not need this much solution, or you may need more. Whatever you do, keep the ratio the same. 1 part vinegar, 7.5 parts distilled water)
Obviously, you'll be washing you hands thoroughly beforehand, and doing this in a clean environment, with only new and sterile equipment. Unless you'd like an abscess from injecting contaminated garbage into yourself.
While this mixture will last a long time at room temperature, it won't hurt to keep it stored in the fridge.
Most of this information was paraphrased from a post by Hulk6767 over at anabolex, so props to him. See the original post:
http://www.anabolex.com/forums/showthread.php?t=48923&highlight=acetic+acid
And the best thing about .6% acetic acid? You can make it yourself:
Things you will need
distilled 5% white vinegar (grocery store, yes, the stuff you put on fish and chips)
distilled water (grocery store, pharmacy)
Alcohol prep pads – sterile kind (70% isopropyl alcohol, pharmacy)
Equipment:
two .2-.22 sterile syringe filters (whatman and millipore are common types)
10mL syringe with a luer lock tip (smaller syringes can obviously work, though you'll be drawing more frequently)
1 standard sterile new syringe
2 new and sterile 20-22 gauge needles
1 empty Sterile glass vial (10-20mL)
Procedure: Alot of this is common knowledge, but here it is anyway
1. swab the top of the empty sterile vial with the alcohol pre pad
2) put the syringe filter on, then attach the needle to this filter. draw up 7.5ml distilled water, then 1ml of vinegar
3) dispose of this needle and filter, attach a new syringe filter, attach the needle onto this filter (making sure not to touch the end of the filter or the exposed needle with your fingers)
4) Inject the 8.5mL of solution into the sterile vial
5) the syringe filter ensures that the mixture you've put in that vial, and
eventually into your body, will be sterile. Using a filter for drawing AND for injecting might be overkill, but it never hurts to be cautious.
5) you now have 8.5ml of of .6 acetic acid
The Reconstitution itself
1) get the regular new sterile syringe
2) draw up the desired amount of .6% aa, which would depend on how strong you want your IGF (read: mcg/ml or mcg/iu)
3) swab the top of the vial containing the IGF
4) inject the mixture SLOWLY down the side of the vial (never directly onto the powder itself!)
5) gently swirl the mixture around until uniform
6) There, that's it.
Notes:
You might not need this much solution, or you may need more. Whatever you do, keep the ratio the same. 1 part vinegar, 7.5 parts distilled water)
Obviously, you'll be washing you hands thoroughly beforehand, and doing this in a clean environment, with only new and sterile equipment. Unless you'd like an abscess from injecting contaminated garbage into yourself.
While this mixture will last a long time at room temperature, it won't hurt to keep it stored in the fridge.
Most of this information was paraphrased from a post by Hulk6767 over at anabolex, so props to him. See the original post:
http://www.anabolex.com/forums/showthread.php?t=48923&highlight=acetic+acid